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. 2020 May 7;5(9):e132747. doi: 10.1172/jci.insight.132747

Figure 1. GPx4 is downregulated and LP levels increase in the myocardium of DIC mice.

Figure 1

(A) Echocardiographic images of CTL mice or DIC mice at day14. (B) LVEF, left ventricular ejection fraction (n = 4). (C) Body weight at day 14 (n = 4). (D) Heart weight (HW), normalized by tibial length (TL) at day 14 (n = 4). (E) Left ventricle (LV) weight, normalized by TL at day 14 (n = 4). (F) Total (left) and mitochondrial (right) Gpx4 expression in the myocardium at day 14 was quantified by real-time PCR (n = 3 and 5, respectively). (G) Western blot of GPx4 from heart tissue lysates at day 14 (n = 6, each). (H) Western blot of mitochondrial lysates obtained from the heart at day 14, for GPx4 (n = 6–7). (I) Western blot of acrolein in heart tissue lysates at day 14 (n = 6). (J) Malondialdehyde (MDA) levels in the myocardium at day 14 were measured by thiobarbituric acid reactive substances (TBARs) assay (n = 3 and 5). (K) MDA levels in the cytosolic fraction (left) and mitochondrial fraction (right) of the myocardium (n = 4 and 9, respectively). (L) Interstitial fibrosis in the LV and Masson trichrome staining in CTL and DIC mice. Scale bars: 50 μm (n = 3). (M) TUNEL staining in CTL and DIC mice at the low power (LP) (scale bar: 50 μm) and the high power (HP) (scale bar: 10 μm), and quantification of the number of TUNEL+ cells (n = 3). Arrowheads indicate TUNEL+ nuclei. Data are shown as the mean ± SEM. Statistical significance was determined using 2-tailed Student’s t test. *P < 0.05, **P < 0.01.