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. 2020 Apr 22;19(5):e13145. doi: 10.1111/acel.13145

FIGURE 3.

FIGURE 3

EGF treatment induced senescent phenotypes in IMR90 cells and HUVECs. (a) IMR90 cells were treated with different concentrations of EGF for 3 days, and the percentages of SAHF‐ and β‐gal‐positive cells were analyzed. (b) Representative pictures of DAPI and SA‐β‐gal staining on IMR90 cells with or without EGF treatment at the indicated concentrations for 3 days. (c) Representative pictures of BrdU staining on IMR90 cells treated with or without EGF treatment at the indicated concentrations for 3 days. (d) Statistical analysis of c. (e) The growth curves of IMR90 cells treated with or without EGF treatment at the indicated concentrations. (f) HUVECs were treated with different concentrations of EGF for 3 days, and the percentages of SAHF‐ and β‐gal‐positive cells were analyzed. (g) Representative pictures of DAPI and SA‐β‐gal staining on HUVECs with or without EGF treatment at the indicated concentrations for 3 days. (h) Representative pictures of BrdU staining on HUVECs with or without EGF treatment at the indicated concentrations for 3 days. (i) Statistical analysis of h. (j) The growth curves of HUVECs treated with or without EGF at the indicated concentrations. Data indicate the mean values calculated from three independent experiments (±SD)