Table 3.

Chikungunya RT-qPCR results for direct vs. RDT RNA extract

Virus dilution	Direct RNA extract			RDT RNA extract
	Cq	Mean Cq	SD of Cq	Cq	Mean Cq	SD of Cq
10−4	22.04	21.77	0.37	23.95	23.97	0.32
	21.35			24.29
	21.93			23.66
10−5	24.95	25.07	0.14	28.04	27.81	0.29
	25.03			27.48
	25.23			27.91
10−6	28.20	28.22	0.60	31.08	31.19	0.52
	27.64			31.76
	28.83			30.74
10−7	32.15	32.03	0.15	33.96	34.21	0.51
	32.08			34.79
	31.86			33.87
10−8	34.58	34.99	0.40	36.98	No Cq	–
	35.37			37.72
	35.01			No Cq
10−9	36.95	37.78	1.06	No Cq	No Cq	–
	38.97			No Cq
	37.41			No Cq

Cq = quantification cycle value; RDT = rapid diagnostic test; RT-qPCR = reverse transcription real-time polymerase chain reaction. All RT-qPCR results displayed in this table are from same PCR run. Limit of detection: lowest virus dilution positive by RT-qPCR tested in triplicate is shaded in gray.