Table 4.

Zika RT-qPCR results for direct vs. RDT RNA extract

Virus dilution	Direct RNA extract			RDT RNA extract
	Cq	Mean Cq	SD of Cq	Cq	Mean Cq	SD of Cq
10−2	20.40	20.63	0.25	25.50	25.30	0.20
	20.89			25.30
	20.61			25.10
10−3	24.15	24.35	0.27	28.68	29.04	0.36
	24.65			29.05
	24.24			29.40
10−4	27.71	27.68	0.24	31.59	31.41	0.16
	27.90			31.35
	27.42			31.29
10−5	31.50	31.36	0.21	33.55	33.83	0.37
	31.45			33.68
	31.12			34.25
10−6	34.62	34.76	0.13	37.44	37.44	0.47
	34.79			37.91
	34.88			36.98
10−7	38.43	39.98	2.44	No Ct	No Cq	–
	42.79			No Ct
	38.71			38.99
10−8	43.78	No Cq	–	44.01	No Cq	–
	39.77			No Ct
	No Ct			No Ct

Cq = quantification cycle value; RDT = rapid diagnostic test; RT-qPCR = reverse transcription real-time polymerase chain reaction. All RT-qPCR results displayed in this table are from same PCR run. Limit of detection: lowest virus dilution positive by RT-qPCR tested in triplicate is shaded in gray.