Table 2.
Performance of the 5-Plex to identify P. falciparum using Pf LDH and Pan LDH assays with hrp2/3–deleted P. falciparum samples
| Category | hrp2/3 deletion by polymerase chain reaction | 5-Plex, positive sample number. | % Accuracy | ||||
|---|---|---|---|---|---|---|---|
| Sample number | HRP2 | Pf LDH | Pv LDH | Pan LDH | Pf classification | hrp2/3 deletion | |
| hrp2+/hrp3+ | 5 | 5 | 5 | 0 | 5 | 100 | NA |
| hrp2+/hrp3− | 6 | 6 | 6 | 0 | 6 | 100 | NA |
| hrp2−/hrp3+ | 3 | 1* | 3 | 0 | 3 | 100 | NA |
| hrp2−/hrp3− | 12 | 3† | 12 | 0 | 12 | 100 | 75.0 |
HRP = histidine-rich protein; NA = not applicable; P. falciparum = Plasmodium falciparum; Pf LDH = P. falciparum-specific lactate dehydrogenase; Pan LDH = Pan malaria lactate dehydrogenase; Pv LDH = P. vivax–specific LDH.
* 230 ng/mL of HRP2 by the 5-Plex; no conventional ELISA data available.
† One sample was detected for 40,000 pg/mL of HRP2 by the 5-Plex but was not quantifiable by the conventional ELISA. Two other samples were 18.9 and 9 pg/mL of HRP2 by the 5-Plex; no conventional ELISA data available.