Figure 2. Transition to more highly mineralised bone during maturation of the metaphysis in female control (Dmp1^Cre) and Dmp1^Cre:Socs3^f/f femora.

(A) Diagram showing region measured, and Otsu thresholding of a raw micro-CT image. (B) Bone volume, as a percentage of total metaphyseal volume, segregated by low, mid and high density volumes, at 12 and 15 weeks in Dmp1^Cre and Dmp1^Cre:Socs3^f/f mice; black asterisks denote significant changes in total bone volume, as indicated by square brackets. Changes in low-, mid- and high-density bone are indicated by coloured asterisks; error bars shown are SEM for the low-, mid- and high-density bone volumes. **, p<0.01; ***, p<0.001 for comparisons shown, determined by repeated measures two-way ANOVA with Šidák post-hoc test; n = 9–11 mice per group.