Table 1. Phenotypic data from 12 week old Dmp1Cre:Il6stf/f mice, including mRNA levels of the targeted gene in flushed femoral samples (Il6st:B2m), femoral trabecular and cortical structure (by micro-computed tomography) and tibial trabecular histomorphometry in the secondary spongiosa.
Data are mean ± SEM. *, p<0.05, **, p<0.01; ***, p<0.001 vs sex- and age-matched controls (Dmp1Cre) by two-way ANOVA with Šidák post-hoc test.
| Parameter | male | female | ||
|---|---|---|---|---|
| Dmp1Cre | Dmp1Cre:Il6stf/f | Dmp1Cre | Dmp1Cre:Il6stf/f | |
| Number of samples | 7 | 10 | 6 | 6 |
| IL6st:B2m (mRNA) | 1.14 ± 0.21 | 0.52 ± 0.08** | 0.88 ± 0.15 | 0.40 ± 0.18 |
| Trabecular bone volume (%) Trabecular number (/mm) Trabecular Thickness (μm) Trabecular Separation (μm) |
13.72 ± 1.26 2.54 ± 0.21 53.5 ± 0.8 217 ± 14.01 |
8.24 ± 0.96** 1.27 ± 0.12*** 64.1 ± 2.6*** 469 ± 26*** |
10.82 ± 1.06 2.38 ± 0.22 45.6 ± 1.0 239 ± 15 |
6.06 ± 0.51** 1.12 ± 0.08*** 54.0 ± 1.3*** 415 ± 29*** |
| Periosteal Perimeter (mm) Marrow Area (mm2) Moment of Inertia (mm4) |
7.21 ± 0.14 1.10 ± 0.04 0.395 ± 0.021 |
8.14 ± 0.25*** 1.37 ± 0.08*** 0.566 ± 0.51*** |
6.78 ± 0.13 0.94 ± 0.04 0.344 ± 0.02 |
7.29 ± 0.10* 1.11 ± 0.02* 0.404 ± 0.009 |
| Bone Formation Rate/Bone Surface (%) Double labelled Surface/Bone Surface (%) Mineral Appositional Rate (μm/day) Osteoclast Surface/Bone Surface (%) |
0.454 ± 0.019 29.1 ± 1.8 1.30 ± 0.08 4.39 ± 0.58 |
0.295 ± 0.048** 20.1 ± 2.8** 1.10 ± 0.14 6.59 ± 0.89 |
0.704 ± 0.03 26.6 ± 1.5 1.98 ± 0.24 3.61 ± 0.45 |
0.481 ± 0.065* 15.1 ± 1.8** 1.63 ± 0.19 4.82 ± 0.83 |