Table 2. mRNA analysis of osteoclast-related genes in flushed femora from 12 week old Dmp1Cre:Socs3f/f and Dmp1Cre:Socs3f/f: Il6stf/f.
Data are mean ± SEM normalised to the geometric mean of B2m and Hprt1; p values from two-way ANOVA analysis are shown in the right column, including p values for the comparison of male vs female, Dmp1Cre:Socs3f/f and Dmp1Cre:Socs3f/f:Il6stf/f (genotype) and the interaction between the two. No statistical differences between genotypes were detected by Šidák post-hoc test within each sex.
| Parameter | male | female | p values | ||
|---|---|---|---|---|---|
| Dmp1Cre:Socs3f/f | Dmp1Cre:Socs3f/f:Il6stf/f | Dmp1Cre:Socs3f/f | Dmp1Cre:Socs3f/f:Il6stf/f | ||
| Number of samples | 10 | 8 | 10 | 8 | |
|
Tnfsf11 (RANKL) Tnfrsf11b (OPG) Acp5 (TRAP) |
0.015 ± 0.002 0.067 ± 0.014 3.64 ± 0.36 |
0.016 ± 0.004 0.097 ± 0.017 1.94 ± 0.26 |
0.015 ± 0.003 0.026 ± 0.004 10.25 ± 1.45 |
0.015 ± 0.003 0.044 ± 0.009 7.41 ± 1.12 |
Male vs female: 0.752; Genotype: 0.841; Interaction: 0.923. Male vs female: 0.0003; Genotype: 0.048; Interaction: 0.635. Male vs female:<0.0001; Genotype: 0.028; Interaction: 0.568. |