Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Biotinylated 6x-His Tag monoclonal antibody | Invitrogen | Cat# MA1-21315-BTIN | Molar ratio of 2:1 (antibody:bMRP1) |
| Cell line | Sf9 | ATCC | CRL-1711 | |
| Cell line | HEK293S GnTI- | ATCC | CRL-3022 | |
| Recombinant DNA reagent | bovine MRP1 in pUC57 vector | Bio Basic | Codon-optimized | |
| Recombinant DNA reagent | bovine MRP1 in a modified pEG BacMam vector | Johnson and Chen, 2017 | Suitable for expression in mammalian cells | |
| Recombinant DNA reagent | bovine MRP1 with S6/A1 peptides for site-specific labeling | This paper | ||
| Recombinant DNA reagent | bovine MRP1 E1454Q with S6/A1 peptides for site-specific labeling | This paper | ||
| Recombinant DNA reagent | Sfp pet29b C-terminal His Tag | (Worthington and Burkart, 2006) | Addgene Plasmid# 75015 | |
| Recombinant DNA reagent | pET15b-ACPs (from S. pneumoniae) | Gift from Michael Johnson | Addgene Plasmid# 63687 | |
| Chemical compound, drug | Cy3 maleimide mono-reactive dye | GE Healthcare | Cat# PA23031 | |
| Chemical compound, drug | LD655 maleimide mono-reactive dye | Lumidyne Technologies | Cat# LD655-MAL | |
| Chemical compound, drug | Trolox | Sigma-Aldrich | Cat# 238813 | |
| Chemical compound, drug | 4-Nitrobenzyl alcohol (NBA) | Sigma-Aldrich | Cat# N12821 | |
| Chemical compound, drug | Cyclooctatetraene (COT) | Sigma-Aldrich | Cat# 138924 | |
| Chemical compound, drug | 3,4-Dihydroxybenzoic acid (PCA) | Sigma-Aldrich | Cat# 37580 | |
| Chemical compound, drug | Digitonin | Sigma-Aldrich | Cat# D141 | |
| Peptide, recombinant protein | Protocatechuate 3,4-Dioxygenase (PCD) | Sigma-Aldrich | Cat# P8279 | |
| Peptide, recombinant protein | Leukotriene C4 | Cayman Chemical | Cat# 20210 | |
| Commercial assay, kit | NHS-activated Sepharose 4 Fast Flow resin | GE Healthcare | Cat# 17-0430-01 | |
| Commercial assay, kit | Superose 6, 10/300 GL | GE Healthcare | Cat# 17-5172-01 | |
| Software, algorithm | SPARTAN | (Juette et al., 2016) | https://www.scottcblanchardlab.com/software | |
| Software, algorithm | ebFRET | (van de Meent et al., 2014) | http://ebfret.github.io | |
| Software, algorithm | MATLAB | MathWorks | https://www.mathworks.com/products/matlab.html | |
| Software, algorithm | Origin | OriginLab | https://www.originlab.com | |
| Software, algorithm | GraphPad Prism | GraphPad | https://www.graphpad.com/scientific-software/prism/ | |
| Software, algorithm | RELION 1.4 | (Scheres, 2012) | https://www2.mrc-lmb.cam.ac.uk/relion | |
| Software, algorithm | Frealign | (Grigorieff, 2016) | https://grigoriefflab.janelia.org/frealign | |
| Software, algorithm | Coot | (Emsley and Cowtan, 2004) | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot | |
| Software, algorithm | PHENIX | (Adams et al., 2010) | https://www.phenix-online.org | |
| Software, algorithm | REFMAC | (Brown et al., 2015) | https://www.ccp4.ac.uk/html/refmac5.html | |
| Software, algorithm | MolProbity | (Chen et al., 2010) | https://molprobity.biochem.duke.edu | |
| Software, algorithm | Chimera | (Pettersen et al., 2004) | https://www.cgl.ucsf.edu/chimera | |
| Software, algorithm | PyMOL | PyMOL | https://www.pymol.org | |
| Software, algorithm | cryoSPARC | (Punjani et al., 2017) | https://cryosparc.com | |
| Other | R1.2/1.3 400 mesh Au holey carbon grids | Quantifoil | Cat# 1210627 | 1 µg/mL |