Table 2. Summary of EM data and structure refinement statistics.
| Data collection | ||
|---|---|---|
| Microscope | Titan krios (FEI) | |
| Voltage (kV) | 300 | |
| Detector | K2 Summit (Gatan) | |
| Pixel size (Å) | 1.03 | |
| Defocus range (μm) | 0.7 to 2.4 | |
| Movies | 3604 | |
| Frames/movie | 50 | |
| Dose rate (electrons/pixel/s) | 8.0 | |
| Total dose (electrons/Å2) | 75 | |
| Number of particles | 1,143,729 | |
| Model composition | ||
| Non-hydrogen atoms | 9684 | |
| Protein residues | 1210 | |
| Lipids/Detergents/Ligands | 3 CHS/1 ATP/1 ADP/2 Mg2+ | |
| Refinement | ||
| Resolution (Å) | 3.23 | |
| Rwork | 0.265 | |
| Rfree | 0.276 | |
| RMS deviations | ||
| Bond lengths (Å) | 0.003 | |
| Bond angles (°) | 1.297 | |
| Validation | ||
| Molprobity score | 1.11 | |
| Clashscore, all atoms | 1.07 | |
| Favored rotamers (%) | 97.7 | |
| Ramachandran plot (%) | ||
| Favored | 95.7 | |
| Allowed | 4.3 | |
| Outliers | 0.0 | |