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Isolated PMNs labeled with Hoechst (blue) and cell-impermeable Sytox Green were perfused over αIIbβ3-coated microchannels at 50 s−1 for 10 min and then monitored under static conditions. Video shown represents period from 80 to 95 min after attachment. Sytox Green staining appears, indicative of DNA becoming extracellular highlights the neutrophils undergoing NETosis.
