Video 7. Neutrophil binding to VWF-‘primed’ platelets occurs via SLC44A2 is modified by the rs2288904 SNP.
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Plasma-free blood generated from individuals homozygous for the rs2288904-G major allele in SLC44A2 (SLC44A2 (R154/R154)) or the rs2288904-A minor allele (SLC44A2 (Q154/Q154)) SNP in SLC44A2 was labeled with DiOC6 and perfused through channels coated with VWF at high shear for 3.5 min. Shear was subsequently reduced to 50 s−1. SLC44A2 (R154/R154) leukocytes (tracked in blue) were seen to roll on the VWF-‘primed’ platelets. The number of leukocytes interacting was severely reduced in the presence of the anti-SLC44A2 #2 antibody. Leukocytes homozygous for the rs2288904-A minor allele, SLC44A2 (Q154/Q154), associated with protection against venous thrombosis, exhibited reduced ability to interact with VWF-‘primed’ platelets.