Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | XL10 Gold | Agilent | 200314 | Chemically competent cells for DNA propagation |
| Recombinant DNA reagent | pASK-CLC-ec1 (plasmid) | PMID:14718478 | Plasmid containing CLC-ec1 | |
| Chemical compound, drug | n-Decyl-β-D-maltoside | Anatrace | D322 | High-purity detergent for protein purification |
| Chemical compound, drug | Lauryl Maltose Neopentyl Glycol (LMNG) | Anatrace | NG310 | High-purity detergent for protein purification |
| Chemical compound, drug | E. coli polar lipid extract | Avanti Polar lipids | 100600C | Lipids for protein reconstitution |
| Chemical compound, drug | Isethionic acid | Wako Chemicals | 350–15765 | Acid form of isethionate, used in transport assays |
| Chemical compound, drug | 1-Oxyl-2,2,5,5,-tetramethylpyrroline-3-methyl methanethio-sulfonate | Fisher Scientific | NC9859662 | Spin label for DEER spectroscopy |
| Sequence-based reagent | forward sequencing primer, pASK/CLC-ec1 | IDT | 5’-CCACTCCCTATCAGTG-3’ | |
| Sequence-based reagent | forward sequencing primer, CLC-ec1 internal | IDT | 5’-GGTGTCATTATGTCGACCATTATGTACCGG-3’ | |
| Software algorithm | XDS | PMID:20124692 | RRID:SCR_015652 | Data Processing |
| Software algorithm | AIMLESS | PMID:16369096 | RRID:SCR_015747 | Data Processing |
| Software algorithm | PHASER | PMID:19461840 | RRID:SCR_014219 | Structure determination |
| Software algorithm | Coot | PMID:20383002 | RRID:SCR_014222 | Structure refinement |
| Software algorithm | REFMAC | PMID:15299926 | RRID:SCR_014225 | Structure refinement |
| Software algorithm | HOLE | PMID:9195488 | Structure analysis | |
| Software algorithm | Caver | PMID:23093919 | Structure analysis | |
| Software algorithm | Pymol | Pymol | RRID:SCR_000305 | Structure analysis |
| Software algorithm | Adobe Illustrator | Adobe | RRID:SCR_010279 | Figure generation |
| Software algorithm | Microcal Origin | Microcal | RRID:SCR_002815 | ITC measurements |
| Software algorithm | pClamp 9.0 | Molecular Devices | RRID:SCR_011323 | Transport assays |
| Software algorithm | Igor Pro | Wavemetrics | RRID:SCR_000325 | Transport assays, difference distance matrices |
| Software algorithm | Matlab | Mathworks | RRID:SCR_001622 | DEER analysis |
| Software algorithm | PROPKA | PMID:26596171
PMID:21269479 |
MD simulations | |
| Software algorithm | VMD | PMID:8744570 | RRID:SCR_001820 | MD simulations |
| Software algorithm | DOWSER | PMID:9162944
PMID:25328496 |
MD simulations | |
| Software algorithm | CHARMM-GUI Membrane Builder | PMID:25130509 | MD simulations | |
| Software algorithm | NAMD2.12 | PMID:16222654 | MD simulations |