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Manual scoring of mRNA-ISH assay. Relationship between manual ISH score (mRNA-ISH) and H-score (IHC). A, ER antibody clone SP1. B, ER antibody clone 6F11. For both clones a manual ISH score of 0 (<1 dot/10 cells) corresponds to an IHC H-score <10. *The case from Figure 1B (H-score=96) indicating a false-positive IHC staining by the clone 6F11.
