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. 2020 Apr 23;40(6):1559–1573. doi: 10.1161/ATVBAHA.120.314297

Figure 3.

Figure 3.

Inflammatory monocyte/macrophage invasion and MMP (matrix metalloprotease)-9 activity were enhanced in EP4 (prostaglandin E receptor 4)-Tg aorta at initial stage of abdominal aortic aneurysm formation. A, Representative fluorescence-activated cell sorting (FACS) plots of cell suspensions obtained from the abdominal aorta before and after Ang II (angiotensin II) infusion. B, Percentage of each cell fraction from FACS analysis. n=9. C and D, Representative image of gelatin zymography of cell suspensions obtained from the abdominal aorta for nontransgenic (non-Tg; N) and EP4-Tg (E) before and after Ang II infusion. Protein expression of pro-MMP-9 and activity of MMP from gelatin zymography. P: recombinant MMP-9 as a positive control. n=6 to 7. E, Semiquantitative polymerase chain reaction of cell fraction from FACS analysis. CD indicates cluster of differentiation; FSC, forward scatter; and NS, not significant. *P<0.05, **P<0.01.