Table 2.
Summary of the Mutations in the Isolated Clones of Shewanella oneidensis MR-1 (pBP136Gm) Evolved in Chemostats.
| Replicon | Gene | C1I17 | C1I25 | C3I1 | C3I5 | C1I27 | C1I16a | C1I26 | C1I12 | Position | Mutation | Description |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chromosome (NC 004347) | SO0569 | x | 590478 | C>T (G39D) | Diguanylate cyclase | |||||||
| cheC | xb | 590787 | C>T (R274H) | Chemotaxis signal transduction system | ||||||||
| SO1278>/<SO1281 | x | 1332264 | +ATGCCATCTCTG | Chemotaxis signal transduction system methyl accepting sensory transducer/hypothetical protein | ||||||||
| SO1699 | x | 1789463 | A>T (L127Q) | Transmembrane transcriptional regulator | ||||||||
| phoP | x | 2049619 | C>A (G59C) | Two component signal transduction system response regulator | ||||||||
| wbpA | xb | 3328917 | tnpA c | UDP-N-acetyl-d-glucosamine 6-dehydrogenase | ||||||||
| cheR | xb | xb | xb | 3388924 | tnpA c | Chemotaxis signal transduction system MCP methyltransferase | ||||||
| xb | 3389346 | G>A (A111V) | ||||||||||
| xb | xb | 3389372 | tnpA c | |||||||||
| metB | xb,d | xb,d | 4212022 | C>A (A137A) | Cystathionine gamma-synthase | |||||||
| MxdA</<SO4181 | xb | xb | 4354008 | (A)6>7 | Diguanylate cyclase-like protein/RNA pseudouridylate synthase family protein | |||||||
| x | x | xe | 4354022 | (A)5>6 | ||||||||
| pMR-1L | SOA0112; SOA0115 | x | 91763 | C>G (R1384G) | Putative lipoprotein | |||||||
| x | 91703 | G>T (D2775Y) | ||||||||||
| Total number of mutations | 4 | 2 | 3 | 3 | 2 | 3 | 2 | 2 | ||||
a
Gray shading highlights the evolved clones with no change in plasmid persistence.
b
Mutation detected by the whole-population sequencing approach.
c
The insertion of a transposable element at that position.
d
Silent mutation.
e
Detected in the marginal prediction.