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. 2020 Mar 24;175(2):168–181. doi: 10.1093/toxsci/kfaa041

Figure 4.

Figure 4.

Effect of netrin-1 on acetaminophen (APAP)-induced macrophage infiltration and liver regeneration. Fasted C57BL/6J mice were cotreated with 300 mg/kg APAP and netrin-1 (1 µg/mouse) or saline as control. Liver tissue was obtained at 6 and 24-h post-APAP. A, Immunohistochemistry staining for F4/80-positive macrophages (×50 magnification). Panels on the extreme right indicate enlargements of indicated areas from 24 h sections. B, Serial sections from 24 h APAP+netrin-1-treated mice probed for adenosine A2B or F4/80 as a marker of macrophages by immunohistochemistry (×50 magnification). Panels on the right are enlarged from areas marked on the left. C, Immunostaining for PCNA in liver sections obtained 24-h post-APAP or APAP+netrin-1 (×50 magnification).