Fig. 4.

CRISPR/Cas9 directed dsx crispant phenotypes in Bicyclus anynana. (A) Schematic of dsx male and female isoforms with the region targeted by CRISPR/Cas9 marked with a red arrow. In panels B–H, wildtype (WT) is shown to the left and crispants are indicated in red to the right. (B–E) Mosaic phenotypes on male (B, C) ventral forewing patches and broad silvery scale region and (D, E) dorsal hindwing patches. Black dotted lines in (B) highlight homologous veins and in (C) outlines patch 16. Red arrowheads indicate some of the mosaic phenotypes. (F) dsx crispant phenotypes on female ventral forewings (top) and dorsal hindwings (bottom). Red arrowheads indicate the differences in length of the white band between WT and crispant. (G) Intersex phenotypes of male genitalia with reduced claspers, aedeagus (bottom) and an intermediate morphology of the uncus (red arrowhead). (H) Intersex phenotypes of female genitalia. Intermediate structures are indicated with red arrowheads.