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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Hepatology. 2020 May 22;72(2):642–655. doi: 10.1002/hep.31048

Figure 4: Combined global Hfe and endothelial Bmp2 KO worsens hepcidin deficiency and iron overload compared to single KO mice.

Figure 4:

Eight-week-old littermate female (closed symbols) and male (open symbols) control (Hfewt/wt;Bmp2fl/fl;Tek-Cre-), single endothelial Bmp2 KO (Hfewt/wt;Bmp2fl/fl;Tek-Cre+), single global Hfe KO (Hfe−/−;Bmp2fl/fl;Tek-Cre-), and double endothelial Bmp2 and global Hfe KO (Hfe−/−;Bmp2fl/fl;Tek-Cre+) mice were analyzed for (A) liver Hamp relative to Rpl19 mRNA by qRT-PCR. The average of the control female mice was set to 1. Hamp/Rpl19 mRNA was divided by liver iron content to normalize for the degree of iron overload in the right panel. (B) Serum iron, serum transferrin saturation and tissue iron levels in the (C) liver, (D) spleen, (E) pancreas, and (F) heart were analyzed by colorimetric assay. For all graphs, individual data points are shown and bars represent mean ± SEM. Female and male mice were analyzed separately by one-way ANOVA with Tukey’s post-hoc test. Means without a common superscript differ significantly (P < 0.05).