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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2020 Apr 4;510:110814. doi: 10.1016/j.mce.2020.110814

Figure 3: Representative individual HUVEC Ca2+ tracings before and after CLA Mix pretreatment and Control, VEGF165, or TNFα treatment.

Figure 3:

Panels A, C, and E show representative HUVEC ([Ca2+ ]i) tracings with a short baseline followed by 100uM ATP stimulation (at time 0 sec), resulting in initial ([Ca2+ ]i) peak followed by repetitive Ca2+ bursts. Panel B shows the same cell as panel A, with addition of a 10um CLA mix pretreatment for 30 minutes before vehicle control treatment for 30 minutes and subsequently stimulated with ATP (at time 0 sec). Panel D shows the same cell as Panel C, with the addition of 10um CLA mix pretreatment for 30 minutes before 10ng/ml VEGF165 for 30 minutes and subsequent stimulation with ATP (at time 0 sec). Likewise, Panel F shows the same cell as in Panel E, with the addition of 10um CLA mix pretreatment for 30 minutes before 10ng/ml TNFα for 60 minutes and subsequent stimulation with ATP (at time 0 sec). An improvement in the degree of lost bursting as seen in Figure 1 established CLA-mediated rescue of Ca2+ bursting function.