Phospho- specific western blot analysis for Y265 on Cx43 were done and representative blots are shown. HUVEC were pretreated with CLA isomers or a 1:1 mix for 30 minutes prior to TNFα (1 hr, panel A) or VEGF165 (30 min, panel B) stimulation. Each band was normalized to Hsp90 loading control and expressed as fold of unstimulated control. In panel C, values are depicted as mean ± S.E.M. with a minimum of 6 independent experiments. Statistical significance is indicated by * p<0.01 vs unstimulated control, # p<0.05 vs unstimulated control, and + p<0.01 vs treatment alone by post-hoc Turkey HSD one-way ANOVA with Holm interference.