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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2020 Apr 4;510:110814. doi: 10.1016/j.mce.2020.110814

Figure 6: TNFα and VEGF165 promote phosphorylation of Cx43 at residue Y265 and TNFα stimulated phosphorylation is reversed by t10,c12 CLA.

Figure 6:

Phospho- specific western blot analysis for Y265 on Cx43 were done and representative blots are shown. HUVEC were pretreated with CLA isomers or a 1:1 mix for 30 minutes prior to TNFα (1 hr, panel A) or VEGF165 (30 min, panel B) stimulation. Each band was normalized to Hsp90 loading control and expressed as fold of unstimulated control. In panel C, values are depicted as mean ± S.E.M. with a minimum of 6 independent experiments. Statistical significance is indicated by * p<0.01 vs unstimulated control, # p<0.05 vs unstimulated control, and + p<0.01 vs treatment alone by post-hoc Turkey HSD one-way ANOVA with Holm interference.