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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2020 Apr 4;510:110814. doi: 10.1016/j.mce.2020.110814

Figure 9: The 10uM CLA mix rescues ATP-stimulated Ca2+ bursts only under Medium and High levels of VEGF165 and TNFα.

Figure 9:

Normal pregnancy (30 minutes 0.5ng/ml VEGF165 and 0.03ng/ml TNFα) and PE-mimicking combination pretreatments (30 minutes) of varying doses of VEGF165 and a single dose of TNFα ((L)PE V+T: 0.1ng/ml VEGF165 and 0.5ng/ml TNFα; (M)PE V+T: 1ng/ml VEGF165 and 0.5ng/ml TNFα; (H)PE V+T: 10ng/ml VEGF165 and 0.5ng/ml TNFα) inhibited ATP-stimulated Ca2+ bursts in a dose-dependent manner. A 30 minute pretreatment with the 10uM 1:1 mix of CLA isomers exacerbated the inhibition due to the Normal V+T, (L)PE V+T combination, but rescued ATP-stimulated Ca2+ bursts for the (M)PE V+T and (H)PE V+T combinations. Data is presented as mean Ca2+ burst numbers for those cells that showed 3+ bursts on initial ATP treatment ± SEM on a minimum of 75 cells from at least 4 separate dishes. Significance by Rank-sum test is indicated as p<0.05 for * Control vs Treatment (w/ or w/o CLA); + Significant improvement after CLA pretreatment vs Treatment; # Significant exacerbation of Ca2+ burst inhibition by CLA pretreatment vs Treatment. Typical physiological range is shown with shaded region as defined in (Boeldt et al., 2017,Bird et al., 2013), adjusted to HUVEC.