TABLE 1.

Mesor, amplitude, and acrophase of mRNA levels of clock genes in the livers of control, HFD, and MelHF mice^a

Gene	Group	Acrophase (h)	Mesor	Amplitude	P value
Clock	Cont	0.59	0.61	0.21	<0.01
	HFD				ns
	MelHF	6.57	0.41	0.16	<0.05
Cry1	Cont	1.90	0.85	0.37	<0.01
	HFD	1.12	0.82	0.27	<0.01
	MelHF	0.54	0.71	0.27	<0.01
Cry2	Cont	20.42	0.97	0.56	<0.01
	HFD	20.63	0.83	0.40	<0.01
	MelHF	17.84	0.64	0.30	<0.01
Per1	Cont	19.60	2.88	4.20	<0.01
	HFD	19.30	2.44	3.30	<0.01
	MelHF	18.38	1.90	2.37	<0.01
Per2	Cont	22.27	0.60	0.5	<0.01
	HFD	21.91	0.44	0.38	<0.01
	MelHF	20.89	0.30	0.25	<0.01

^aThe rhythmicity was assessed by cosinor analysis, and P < 0.05 indicated a significant rhythm; ns means the difference was nonsignificant (P > 0.05). The model can be written according to the equation f(x) = A + B cos [2 π(x + C)/24], with f(x) indicating relative expression levels of target genes, x indicating the time of sampling (h), A indicating the mean value of the cosine curve (midline estimating statistic of rhythm [mesor]), B indicating the amplitude of the curve (half of the sinusoid), and C indicating the acrophase (h).