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. 2020 May 12;51(3):241–250. doi: 10.1007/s10735-020-09876-7

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© The Author(s) 2020

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Fig. 2 — Knocking down IFT172 impairs MSCs ciliogenesis. Primary cilia were visualized by the combined staining of α-tubulin and γ-tubulin (a, b). The length of the cilia was measured by ImageJ software (c). The stained primary cilia were counted and reported as the number per 1000 nuclei, and the ratio was calculated (d). Scale bars: 20 µM