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. 2020 May 12;51(3):241–250. doi: 10.1007/s10735-020-09876-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — IFT172 and KIF3A siRNA knockdown initiates MSCs differentiation and downregulates pluripotency-related gene expression. Mesenchymal stem cells were cultured in MesenPRO medium for 6 days and treated with siRNA on D1 D3 and D5. qPCR was carried out to quantify the gene expression of the pluripotent transcription factors and endoderm, ectoderm and mesoderm lineage markers. Data on the MSCs from two different MSCs donors are presented from three experiments