Fig. 3.

MT7 elevated phosphorylation of AMPK in a dose and time–dependent manner. a Sensory neurons derived from a 3–5-month STZ-diabetic rat were cultured overnight and then treated with varying doses of MT7 for 1 h. Western blots are shown for P-AMPK, T-AMPK, and T-ERK. b Levels of expression of these proteins presented relative to T-ERK or T-AMPK. c Samples from the experiment in b were probed for P-ACC. d Neurons from a diabetic rat were cultured overnight and then exposed to 100 nM MT7 for varying times. e Levels of P-AMPK presented relative to T-ERK and T-AMPK. For b, c, and e, values are means ± SEM, n = 3 replicate cultures; *p < 0.05 vs control by one-way ANOVA with Dunnett’s post hoc test. f Pharmacological blockade of CaMKKβ prevents MT7-dependent phosphorylation of AMPK. Neurons from a control rat were cultured overnight and then pretreated with 1 μM STO-609 for 2 h and then treated with 100 nM MT7 for 3 h. Levels of P-AMPK presented relative to T-ERK and T-AMPK. Values are means ± SEM, n = 3 replicate cultures; *p < 0.05 vs other groups by one-way ANOVA with Tukey’s post hoc test