Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Seahorse XF cell phenotype test run protocol
| Command | Time (min) | Port | Drug |
|---|---|---|---|
| Calibrate | |||
| Equilibrate | 12 | ||
| Mix (×5)* | 3 | ||
| Measure (×5)* | 3 | ||
| Inject | A | Oligomycin + FCCP | |
| Mix (×5)** | 3 | ||
| Measure (×5)** | 3 | ||
| Inject | B | Hoechst 33342 | |
| End protocol |
*After the equilibration, the baseline step consisted of 5 “mix and measure” cycles**After the injection of Oligomycin + FCCP, 5 “mix and measure” cycles were performed
