Table 3.
Seahorse XF cell mito stress test run protocol
| Command | Time (min) | Port | Drug |
|---|---|---|---|
| Calibrate | |||
| Equilibrate | 12 | ||
| Mix (x 3)* | 3 | ||
| Measure (x3)* | 3 | ||
| Inject | A | Oligomycin | |
| Mix (x3)** | 3 | ||
| Measure (x3)** | 3 | ||
| Inject | B | FCCP | |
| Mix (x3)** | 3 | ||
| Measure (x3)** | 3 | ||
| Inject | C | Rotenone/Antimycin | |
| Mix (x3)** | 3 | ||
| Measure (x3)** | 3 | ||
| Inject | D | Hoechst 33342 | |
| End protocol |
*After the equilibration, the baseline step consisted of 3 “mix and measure” cycles
**After the injection of Oligomycin, FCCP and Rotenone/Antimycin follow 3 “mix and measure” cycles