Fig. 4.

Dose- and time-dependent cytotoxicity induced by α-Syn oligomeric polymorphs. SH-SY5Y cells and the SH-SY5Y^WT-Syn cells (SH-SY5Y cells overexpressing human wild-type α-Syn protein) were exposed to 0.125 to 1.5 μM of different α-Syn oligomers preparations for the indicated times. a, b The effect of different concentrations of α-Syn aggregates on SH-SY5Y cells as measured by LDH release at 16 and 24 h of incubation. c Cell viability of SH-SY5Y cells exposed to different concentrations of α-Syn aggregates for 24 h measured by MTS assay. d, e The effect of α-Syn aggregates on SH-SY5Y^WT-Syn incubated for 16 and 24 h as measured by LDH release. f Cell viability of SH-SY5Y^WT-Syn cells exposed to different concentrations of α-Syn aggregates for 24 h, measured by MTS assay. Data are represented as mean ± SD from three independent experiments. Statistical significance was calculated using two-way ANOVA with Bonferroni post hoc analysis. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001