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. 2020 Jan 9;28(6):783–789. doi: 10.1038/s41431-019-0567-2

Fig. 3. Immunoblot analysis of transiently expressed GFP-tagged CSPα proteins in CAD5 cells.

Fig. 3

Immunodetection using the CSP antibody reveals that the GFP_CSPα_p.(Cys124_Cys133dup) protein is present exclusively in nonpalmitoylated form (CSPα nonpalm; the asterisk (*) refers to the palmitoylated form—CSPα palm). All three GFP_CSPα proteins with the variants p.(Leu115Arg), p.(Leu116del), p.(Cys124_Cys133dup) form high molecular weight aggregates (**) resistant to sodium dodecyl sulfate (SDS) and reducing agents (DTT, βME). The aggregates are soluble only upon initial chemical depalmitoylation by hydroxylamine (NH2OH).