Fig. 1. Derivation of organoids from murine oviduct and OSE.

a For organoid derivation oviducts and ovaries were separated and subjected to collagenase or pronase treatment, respectively. The representative brightfield images of both organoid cultures are shown. Scale bar, 200 μm. The organoid derivation was reproducible over four independent experiments. b Basic medium requirement of oviductal and OSE organoids. OSE organoids are WNT-dependent. Representative images from n = 3 independent experiments. Est—Estrogen, IWP2—inhibitor of WNT protein 2, asterisk—in the WNT inhibitor conditions Rspo1 is withdrawn from the base medium. Scale bar, 2 mm. c Scatter plot presenting chromosome number distribution and mean of three independent lines, based on organoid metaphase spreads (20 spreads per line, three biological replicates, n = 60). Both wild-type organoid lines sustain healthy karyotype during culturing. a–c OSE—ovarian surface epithelium.