Fig. 5. Both origins are able to give rise to HG-SOC-like tumors.

a Transplantation strategy. The clones were transplanted either subcutaneously or orthotopically into the bursa of immunodeficient mice and tumor formation was assessed up to 4 months after organoid injections. Each mouse received two injections with the same clone into the opposite flanks or bursal cavities (n = 2 injections/mouse). b Representative histological overview and close-up images of subcutaneous tumors derived from transplantations with the different triple mutant oviductal (n = 12 tumors) and OSE (n = 7 tumors) clones. H&E, GFP, KI67, and PAX8 stainings are shown. Scale bar, 25 μm. OSE—ovarian surface epithelium; SC—subcutaneous; TBN—Trp53, Brca1, Nf1 mutant; TBP—Trp53, Brca1, Pten mutant.