Table 3.
Sequences targeted by designed sgRNAs.
| Gene | Exon (F—forward, R—reverse) | Targeted sequence |
|---|---|---|
| Trp53 | Exon 3, F | AAGTCACAGCACATGACGG |
| Brca1 | Exon 6, F | GCGTCGATCATCCAGAGCGT |
| Nf1 | Exon 8, F | CCAGGACATCTCCAAGGATG |
| Pten | Exon 6, R | ATATACATAGCGCCTCTGAC |
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