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. 2020 May 27;13:28. doi: 10.1186/s12284-020-00388-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Fluorometric assays of GFP or LUC driven by OsHsp18.0-CI promoter constructs. a Diagram of the constructs of OsHsfB4d and the OsHsp18.0-CI promoter. b GFP fluorescence of the OsHsp18.0-CI promoter DNA construct (OsHsp18.0-CI_Pro:GFP). Coexpression with or without the OsHsfB4d in N. benthamiana. Images were photographed at 2 d after infiltration. Bar = 200 μm. c Quantitative analysis of the fluorescence intensity. The results are presented as the average fluorescence intensity using ZEN software. The experiments were repeated three times. ** indicates significant differences according to Fisher’s least-significant difference test (P < 0.01). d Luciferase assay of the co-expressed OsHsfB4d and the promoters of OsHsp18.0-CI (OsHsp18.0-CI_Pro:LUC). Mock represents the 10 mM MgCl₂ solution