Fig. 7.

OsHsfB4d binds to the perfect HSE motif with the OsHsp18.0-CI promoter. a EMSA using the probe 1 and different amount of recombinant His-tagged OsHsfB4d. 1× represents about 0.53 μg of the purified OsHsfB4d. b OsHsfB4d bind to the perfect HSE cis-element. The OsHsfB4d protein was incubated with the labeled probe to serve as a positive control; mutated probes (Mu) were used as a negative control. 5-, 10-, 50- and 200-fold excesses of unlabeled probes were used for competition. 50- and 200-fold excesses of mutated probes were used for competition. Mutated probe in which the perfect HSE motif 5′- GAAACTTC − 3′ was replaced with 5′- CAAACAAT -3′