Table 1.
Primer sets for amplification of the whole genome of F/98
| Prime name | Prime sequence (5′–3′) of forward primer | Prime sequence (5′-3′) of reverse primer | Length (bp) |
|---|---|---|---|
| PB2 | |||
| PB2-1 | TATTGGTCTCAGGGAGCGAAAGCAGGTC | ACTGCCTTTATCATGCAGTCATC | 1376 |
| PB2-2 | GCAACCGCTATTTTTAGGAAAGC | ATATGGTCTCGTATTAGTAGAAACAAGGTCG | 1276 |
| PB1 | |||
| PB1-1 | AGCAAAAGCAGGCAAACCATTTGAA | GAGGATTGGAGCCCTTCCCACCA | 1984 |
| PB1-2 | ATGATGATGGGCATGTTCAACATG | AGTAGAAACAAGGCATTTTTTCA | 1120 |
| PA | |||
| PA-1 | AGCTAAAGCAGGTACTGATTCAAAA | ATCCATCTTGATTCCGTCAATTC | 1316 |
| PA-2 | GGCACCTGATAAAGTTGACTTTGA | AGTAGAAACAAGGTACTTTTTTGGA | 1138 |
| HA | TATTCGTCTCAGGGAGCAAAAGCAGGGG | ATATCGTCTCGTATTAGTAGAAACAAGGGTGTTTT | 1683 |
| NP | TATTCGTTCTCAGGGAGCAAAAGCAGGGTA | AGTAGAAACAAGGGTATTTTTCTT | 1497 |
| NA | TATTGGTCTCAGGGAGCAAAAGCAGGAGT | ATATGGTCTCGTATTAGTAGAAACAAGGAGTTTTT | 1417 |
| M | TATTCGTCTCAGGGAGCAAAAGCAGGTAG | ATATCGTCTCGTATTAGTAGAAACAAGGTAGTTTT | 982 |
| NS | TATTCGTCTCAGGGAGCAAAAGCAGGGTG | ATATCGTCTCGTATTAGTATAAACAAGGGTGTTTT | 844 |