Table 6.

ELD₅₀ and ELD₅₀ of the progeny strains

Immune status	Tissue	Strains	Mutations						EID50	ELD50
			HA	NA	PB2	NP	NS1	M1
Immunized	Trachea	V1	K131R, A198T, L234Q	Amino acids 67–76 deletion	R318K, R355K	–	–	–	6.9	0
Immunized	Lung	V2	K131R, A168T, A198T, L234Q	Amino acids 67–76 deletion	R318K	–	–	–	7	0
Immunized	Trachea	V3	K131R, N201D, A198T, L234Q	Amino acids 67–76 deletion	–	–	–	–	7.2	0
Immunized	Lung	V4	K131R, A198T, L234Q	Amino acids 67–76 deletion	R318K, R355K	–	–	–	7	0
Immunized	Lung	V5	K131R, A198T, L234Q	Amino acids 67–76 deletion	–	–	–	–	7	0
Nonimmunized	Trachea	N1	A198V, M224K, Q234L, L281F, N285D	–	R318K	V186I, L466I	L77I	F62L	9.5	7.4
Nonimmunized	Trachea	N2	A198V, M224K, Q234L, N285D	–	R318K	V186I, L466I	L77I	F62L	9.6	7.3
Nonimmunized	Lung	N3	A198V, M224K, Q234L, L281F, N285D	–	R318K	V186I, L466I	L77I	F62L	9.1	7.2
Nonimmunized	Lung	N4	E114K, A198V, M224K, Q234L, L281F, N285D	–	R318K	V186I, L466I	L77I	F62L	9.8	7.5
Nonimmunized	Trachea	N5	A198V, M224K, Q234L, L281F, N285D	–	R318K	V186I, L466I	L77I	F62L	9.4	7.4