TABLE 5.
Distribution of proteins (expressed as protein percentage on total peak areas) in Mw areas (A1, A2, A3) and molecular weight ranges of proteins for each LS type, as a result of LoaC capillary electrophoretic analysis on Protein 230 Labchip, before and after fermentation with Weissella cibaria C43-11 or Lactobacillus plantarum ITM21B and in comparison to the uninoculated control (Co).
| Am | W/Am | Q | W/Q | |||||||||||||
| LS DY500_3%S | ||||||||||||||||
| Mw area (kDa) | T0 | Co | ITM21B | C43-11 | T0 | Co | ITM21B | C43-11 | T0 | Co | ITM21B | C43-11 | T0 | Co | ITM21B | C43-11 |
| A1 (14–30) | 40.9 | 56.7 | 96.9 | 88.7 | 25.1 | 27.8 | 40.7 | 40.2 | 20.1 | 41.8 | 100 | 94.3 | 20.4 | 26.2 | 78.9 | 75.4 |
| A2: | ||||||||||||||||
| I (31–42) | 47.8 | 40.1 | 3.1 | 11.3 | 31.4 | 42.9 | 40.7 | 41.7 | 22.1 | 40.6 | 0 | 5.7 | 18.3 | 21.9 | 4.6 | 13.2 |
| II (43–55) | 8.5 | 1.7 | 0 | 0 | 25.0 | 15.7 | 15.6 | 12.8 | 40.0 | 8.2 | 0 | 0 | 43.7 | 28.7 | 3.7 | 4.0 |
| III (56–79) | 2.2 | 1.5 | 0 | 0 | 13.6 | 11.0 | 3.0 | 5.3 | 13.7 | 6.9 | 0 | 0 | 13.6 | 13.7 | 10.0 | 7.4 |
| A3 (>80 kDa) | 0.6 | 0 | 0 | 0 | 4.9 | 2.6 | 0 | 0 | 4.1 | 2.5 | 0 | 0 | 4.0 | 9.5 | 2.8 | 0 |
| Mw range (kDa) | 14–94 | 14–79 | 14–35 | 14–35 | 14–222 | 14–225 | 14–57 | 14–57 | 14–117 | 14–105 | 14–30 | 14–32 | 14–225 | 14–225 | 14–103 | 14–57 |
|
LS DY250_3%S | ||||||||||||||||
| A1 (14–30) | 43.8 | 55.8 | 89.4 | 88.6 | 24.5 | 24.4 | 73.0 | 37.7 | 24.1 | 37.4 | 93.5 | 70.1 | 14.2 | 20.7 | 95.3 | 55.1 |
| A2: | ||||||||||||||||
| I (31–42) | 46.0 | 40.8 | 10.6 | 11.4 | 33.0 | 36.1 | 15.5 | 33.5 | 27.3 | 39.8 | 6.5 | 27.0 | 4.0 | 17.8 | 4.7 | 21.1 |
| II (43–55) | 7.6 | 1.3 | 0 | 0 | 25.1 | 29.8 | 10.7 | 16.4 | 32.3 | 10.7 | 0 | 0 | 48.4 | 34.0 | 0 | 16.5 |
| III (56–79) | 2.0 | 2.1 | 0 | 0 | 13.6 | 5.7 | 1.9 | 6.9 | 11.4 | 9.1 | 0 | 0.9 | 25.5 | 17.0 | 0 | 7.3 |
| A3 (>80 kDa) | 0.6 | 0 | 0 | 0 | 3.8 | 4.0 | 0 | 5.5 | 4.9 | 3.0 | 0 | 2.0 | 7.9 | 10.5 | 0 | 0 |
| Mw range (kDa) | 14–96 | 14–79 | 14–32 | 14–35 | 14–226 | 14–225 | 15–57 | 14–220 | 14–117 | 14–104 | 14–36 | 14–97 | 14–214 | 14–225 | 14–39 | 14–60 |
|
LS DY250_6%S | ||||||||||||||||
| A1 (14–30) | 46.2 | 62.4 | 94.2 | 74.2 | 34.8 | 27.3 | 68.8 | 53.2 | 20.4 | 40.3 | 93.8 | 64.1 | 16.0 | 21.0 | 100 | 53.4 |
| A2: | ||||||||||||||||
| I (31–42) | 42.3 | 33.8 | 5.8 | 25.8 | 21.6 | 33.8 | 16.9 | 26.1 | 21.1 | 41.7 | 6.2 | 33.9 | 13.2 | 18.7 | 0 | 28.0 |
| II (43–55) | 8.8 | 1.7 | 0 | 0 | 21.2 | 24.9 | 10.8 | 14.3 | 42.1 | 8.1 | 0 | 0 | 32.0 | 37.8 | 0 | 12.0 |
| III (56–79) | 2.0 | 2.2 | 0 | 0 | 13.9 | 10.0 | 0 | 0.5 | 13.9 | 7.1 | 0 | 2.0 | 32.1 | 16.2 | 0 | 5.7 |
| A3 (>80 kDa) | 0.7 | 0 | 0 | 0 | 8.5 | 4.0 | 3.5 | 5.9 | 2.5 | 2.8 | 0 | 0 | 6.7 | 6.3 | 0 | 0.9 |
| Mw range (kDa) | 14–96 | 14–79 | 14–43 | 14–41 | 14–217 | 14–219 | 14–123 | 14–130 | 14–117 | 14–105 | 14–37 | 14–57 | 14–230 | 14–220 | 14–21 | 14–88 |