TABLE 3.
Strains, plasmids, and primers
| Strain, plasmid, or primer | Description or sequence (5′→3′) | Source or reference |
|---|---|---|
| E. coli strains | ||
| BW25113 | Wild type | Laboratory collection |
| DM16769 | pdxI::Cm | This study |
| DM16696 | pdxH::Kan | This study |
| DM16698 | pdxH | This study |
| DM16027 | pdxJ::kan (Keio collection, JW2548-KC) | 33 |
| DM16037 | pdxJ | This study |
| DM16713 | pdxJ pdxH::Kan | This study |
| DM16847 | pdxH::Kan pdxI::Cm | This study |
| DM16865 | pdxH::kan pdxI pdxJ | This study |
| AG1/pCA24N-pdxI | ASKA clone, JW1403-AM | 32 |
| Plasmid pCA24N-pdxI | N-terminal His-tagged PdxI expression (ASKA JW1403-AM) | 32 |
| Primers | ||
| pdxH-H1 | ATGTCTGATAACGACGAATTGCAGCAAATCGCGCATCTG | |
| CGCCGTGAATGTGTAGGCTGGAGCTGCTTCG | ||
| pdxH-H2 | TCAGGGTGCAAGACGATCAATCTTCCACGCATCATTTTC | |
| ACGCTGGTCATATGAATATCCTCCTTAG | ||
| pdxI-H1 | ATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTT | |
| AACCGTCTTGTGTAGGCTGGAGCTGCTTCG | ||
| pdxI-H2 | TTATTCTCGCGAAATACCATCCAACGTAGA | |
| CAACACTTCCTCAGAAAGATCATATGAATATCCTCCTTAG | ||
| pdxH-check-fw | CGCATCGTCTTGAATAACTGTCAG | |
| pdxH-check-rv | CACCTTTGCCGGTACACGACTTTTC | |
| pdxI-check-fw | GCAACTCATCCAGTAATCTTGTTTACACC | |
| pdxI-check-rv | GTAAACGTATCCAGCCGCAATTCC |