Figure 7.

Immunofluorescence staining for IBA1, a microglia activation-specific marker. Mice were killed 3 h (top) or 1 week (bottom) postsurgery. Surgeries were either a standard chronic cranial window surgery (left) or a bubble surgery followed by the mounting of a chronic cranial window (right). There is no noticeable increase in IBA1 3 h following surgery, but in both chronic cranial window surgery and bubble surgery preparations there is a similar increase in IBA1 1 week postsurgery. Surgery was done on the left hemisphere in each coronal slice shown. White rectangles indicate the positions of magnified areas (lower half).