TABLE 1.
PCR primers used for RT-qPCR.
| Gene | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) | Expected size of amplicon (bp) | Annealing temperature (°C) | Reference sequence |
| LALBA | TACGGAGGTGTCAGTTTG | TCCTTGAGTGAGGGTTCT | 160 | 60 | NM_001009797 |
| STAT5a | CAACGGCATGTCTGTGTCCT | AGTGGGGCTTGTGATGTTTCTT | 124 | 60 | NM_001009402.2 |
| HSPA9 | TGAAGACTTTGACCAGGCCT | GAACTCTCTGAAGCGCCATG | 100 | 60 | XM_004008840.4 |
| JAK1 | CTACAATGGCGAGATCCCCT | CTCTGGTTGGGGTCGTAGTT | 105 | 60 | XM_015092018.2 |
| XDH | AGGGAACATCATCACAGCCA | GTAGCTGGGGAAGAAGGTGT | 132 | 60 | XM_027966969.1 |
| LPL | TGCCTTACAAAGTCTTCCA | GCCACAGTGCCATACAGA | 106 | 60 | XM_027963889.1 |
| FAM78B | AGGGAAGGGAGAGTGAAAGC | TTCATGCTGACGGAGAACCT | 127 | 60 | XM_004002724.4 |
| LCN2 | AACGTCACCTCCATCCTGTT | GTGTAGCTCCTTATCCCGGG | 127 | 60 | XM_012147566.2 |
| WIPF1 | CGGAGGTGGTGGAAGTTTTG | GTGAAAGGTTTGGCGGATGT | 166 | 60 | XM_027964833.1 |
| PAPLN | GAAGCCAATGACCTCAGCAG | GAGGTAGTATTCGCCACGGA | 134 | 60 | XM_027971986.1 |
| GDF10 | CCGGAAGAAGCAATGGGATG | CAGTAGTAGGCGTCGAAGGA | 120 | 60 | XM_004021551.4 |
| MYOF | CGACCAGAAACCTCCTTCCT | CCACAAAGAGCAGCAGGATC | 123 | 60 | XM_012102564.3 |
| FSCN1 | ACGAAGAGACCGACCAAGAG | CATTGGACGCACGCAGAATA | 197 | 60 | XM_027961638.1 |
| STAT6 | ATCTTCAGTGACAGCAGCCT | CACCATCAAACCACTGCCAA | 120 | 60 | XM_027967569.1 |
| CD4 | ATATGCCGCTCCAGTGCTAT | CAGCCCAGATGACGGATACT | 139 | 60 | XM_012113399.2 |
| MAP3K14 | TCATGGAACTGCTGGAAGGT | CGTTGTCGGCTTTCACATCT | 158 | 60 | XM_004012997.3 |
| IgA | CTCCTGCTCTGCCACCTACC | GCGTCACCAGCTCGTTGT | 137 | 60 | ENSOARG00000008862 |
| IgG | CAAGGTCCACAACAAAGGC | GCGATGTAGTCTGGGTAGAAGC | 171 | 60 | ENSOARG00000009143 |
| PRPF31 | ACAGATGATGGAAGCAGCAA | GGTTGGGAGGATGAAGGAGT | 101 | 60 | XM_004002450.1 |
| CUL11 | AAAAATACAACGCCCTGGTG | CTGAGCCATCTTGGTGACTG | 116 | 60 | XM_004008343.1 |
1PRPF3 and CUL1 were chosen as internal reference genes as suggested by Paten et al. (2014).