TABLE 1.
Human anti-monoclonal antibodies list used in flow cytometry analysis for mesenchymal stem cell markers detection.
Fluorescently-conjugated antibody/localization marker | Brand/code number | Dilution | Incubation |
CD-105/FITC, surface | Milteny Biotec, 130-098-774 | 1:11 | 30′, +4°C |
CD-29/FITC, surface | Milteny Biotec, 130-101-256 | 1:11 | 30′, +4°C |
CD-90/FITC, surface | Milteny Biotec, 130-114-859 | 1:50 | 30′, +4°C |
CD-73/PE, surface PE | Milteny Biotec, 130-111-908 | 1:50 | 30′, +4°C |
CD-45/FITC, surface | Milteny Biotec, 130-110-631 | 1:50 | 30′, +4°C |
HLA-DR/FITC, surface | BD Pharmingen, 555811 | 1:5 | 30′, +4°C |