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. 2020 May 21;11:492. doi: 10.3389/fphys.2020.00492

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Copyright © 2020 Wehrli, Caporale, Langham and Chatterjee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Multi-vascular MRI protocol. The 50-min MRI protocol included a measure of peripheral vascular reactivity (PVR) to cuff-induced ischemia, followed by cerebrovascular reactivity (CVR) and aortic pulse wave velocity (PWV) quantification. PVR was assessed in the femoral vessels via multiple techniques. To stimulate reactive hyperemia the cuff was placed around the upper thigh and inflated for 5 min. CVR to hypercapnia in the form of volitional apnea was measured in the superior sagittal sinus (artwork modified from Caporale et al., 2019; Supplementary Figure B1, with permission from RSNA publisher).