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. 2020 Apr 18;27(6):1527–1532. doi: 10.1016/j.sjbs.2020.04.027

Table 2.

The detection of the standard curve.

1 μg/μL BSA protein standard solution (μL) Lysis buffer solution (μL) AB mixture solution (μL) BCA working fluid (μL) BSA protein content (μg/μL)
0 5 25 200 180
1 4 25 200 180
2 3 25 200 180
3 2 25 200 180
4 1 25 200 180
5 0 25 200 180