Table 2.
1 μg/μL BSA protein standard solution (μL) | Lysis buffer solution (μL) | AB mixture solution (μL) | BCA working fluid (μL) | BSA protein content (μg/μL) |
---|---|---|---|---|
0 | 5 | 25 | 200 | 180 |
1 | 4 | 25 | 200 | 180 |
2 | 3 | 25 | 200 | 180 |
3 | 2 | 25 | 200 | 180 |
4 | 1 | 25 | 200 | 180 |
5 | 0 | 25 | 200 | 180 |