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hEGFRvIII:CD3 bi-scFv binds to EGFRvIII. U87-MG-EGFRvIII and control U87-MG cells were incubated first with hEGFRvIII:CD3 bi-scFv and then with fluorescent Protein-L. Fluorescence was measured using a flow cytometer. The dashed line shows the EC20 value for binding to U87-MG-EGFRvIII. The line of best fit for U87-MG was ambiguous. EC20, 20% of the maximal effective concentration; EGFRvIII, epidermal growth factor receptor variant III.
