Skip to main content
. Author manuscript; available in PMC: 2021 May 21.
Published in final edited form as: J Theor Biol. 2020 Feb 28;493:110222. doi: 10.1016/j.jtbi.2020.110222

Fig. 9.

Fig. 9.

FT-t cells were transfected with siRNA targeted to SCD1 (siSCD1), ACSL4 (siACSL4), non-targeting siRNA (siNTC) or siRNA targeted to both SCD1 and ACSL4 (siSCD1 + siACSL4) for 48 h. a) Levels of SCD1 mRNA following knockdown of SCD1 and ACSL4 individually or together was Quantified by qRT-PCR. b) Levels of ACSL4 mRNA following knockdown of SCD1 and ACSL4 individually or together was quantified by qRT-PCR. c) Cells were treated with 0.5 to 2 μM RSL3 in the presence or absence of 2 μM ferrostatin-1 for 24 h to assess sensitivity to ferroptosis. Cell viability was measured using calcein-AM. **p<3.6E-29 (siNTC vs siSCD1 and siSCD1 vs siSCD1 + siACSL4) d) Cell death was determined using Trypan-blue exclusion. UNT, untreated controls. *p<2.4E-02 (siNTC vs siSCD1 and siSCD1 vs siSCD1 + siACSL4). Represented are means and standard deviations of three independent experiments, each performed using eight technical replicates per point.