Table 1.
Taste stimuli used for CT experiments.
| (mM) | Stimuli | (mM) | |
|---|---|---|---|
| R | 10 KCl + 10 HEPES | NaCl | 10 KCl + 10 HEPES + 100 NaCl |
| R | 10 KCl + 10 HEPES | NaCl + Bz | 10 KCl + 10 HEPES + 100 NaCl + 0.005 Bz |
| R | 10 KCl + 10 HEPES | NaCl + Bz + RTX | 10 KCl + 10 HEPES + 100 NaCl + 0.005 Bz + RTX (0–0.01) |
| R | 10 KCl + 10 HEPES | NaCl + Bz + FII or sub-fractions | 10 KCl + 10 HEPES + 100 NaCl + 0.005 Bz + FII or sub-fractions |
| R | 10 KCl + 10 HEPES | NaCl + SB | 10 KCl + 10 HEPES + 100 NaCl + 0.001 SB |
| R | 10 KCl + 10 HEPES | NaCl + SB + FII | 10 KCl + 10 HEPES + 100 NaCl + 0.001 SB + FII or sub-fractions) |
| R | 10 KCl + 10 HEPES | N + Bz + SB + FII | 10 KCl + 10 HEPES + 100 NaCl + 0.005 Bz + 0.001 SB + FII or sub-fractions |
| R | 10 KCl | MSG + Bz + SB | 10 KCl + 100 MSG + 0.005 Bz + 0.001 SB |
| R | 10 KCl | MSG + Bz + SB + IMP | 10 KCl + 100 MSG + 0.005 Bz + 0.001 SB + 1 IMP |
| R | 10 KCl | MSG + Bz + SB + FII | 10 KCl + 100 MSG + 0.005 Bz + 0.001 SB + FII |
| R | 10 KCl | Control-1 | 300 NH4Cl |
| R | 10 KCl | Control-2 | 300 NaCl |
4-(2-Hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES) was used to buffer the pH of rinse and salt stimuli at pH 6.1. Bz (benzamil); RTX (resiniferatoxin); SB (SB-366791, N-(3-methoxyphenyl)-4-chlorocinnamide). All compounds were obtained from Sigma-Aldrich.