Skip to main content
. 2020 Apr 11;12(4):945. doi: 10.3390/cancers12040945

Figure 2.

Figure 2

Dex promotes neutrophil and macrophage recruitment in acute ulcerative colitis. The relative proportions of macrophages (CD11b+F4/80+), neutrophils (CD11b+Ly6G+), dendritic cells (DCs) (CD11c+), CD4+ T cells (CD3+CD4+), CD8+ T cells (CD3+CD8+) and B cells (CD19+) in single-cell suspensions derived from mice treated with 3% dextran sulfate sodium (DSS) for 5 days in combination with dexamethasone (Dex) or vehicle (phosphate buffer saline; PBS), with daily intraperitoneal (i.p.) injection. (A,C) Single-cell suspensions of colonic tissue from mice were isolated. Representative flow cytometry figures are shown (A), and the data are summarized (C). The blood cells of the mice were analyzed. The CD11b+CD115+ cells were used as a marker of monocytes. A representative flow cytometry figure is shown (B, upper), and the data are summarized (D). The bone marrow cells of mice were analyzed, and a representative flow cytometry figure is shown (B; lower); the data are summarized (E). Representative results are based on one of three or four independent experiments performed with similar results. The data are presented as the mean ± SD (n = 4–8 mice per group). Statistical significance was measured by one-way ANOVA. ***P< 0.001, compared with the indicated groups.