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Chromatograms from preliminary experiments before sample concentration adjustment. Chromatograms show derivatized Skeletonema costatum extracts with a concentration equivalent to 2.5 × 103 cells per µL sample measured on the Orbitrap GC (bottom), with a split ratio of 1:5, and the GC-ISQ (top), with a split ratio of 1:8 (minimal split ratios on each machine).
