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. 2020 May 28;18:56. doi: 10.1186/s12958-020-00596-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Proteomic analysis of testicular tissues. (a) Venn diagram of total described testicular proteins in mice (n = 4) after various treatments in different experimental groups. (b) Projection of 24 experimental mice into the space of first three principal components according to PCA; percentages in the axis legends show the proportion of total variance explained by the particular component. (c) Overview of acetylated and (d) phosphorylated testicular proteins. (e) Analysis of γH2AX; band signals were normalized to α-tubulin and related to the vehicle control as the mean (range) of three independent experiments. Unpaired t tests were performed, and statistical significance is indicated (*p < 0.05). VC: vehicle control, BPS1–3: increasing doses of bisphenol S